nsclc cell lines sw1573 Search Results


2170 2r  (ATCC)
95
ATCC 2170 2r
2170 2r, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pmc11274128-276-10-13?v=ATCC
Average 95 stars, based on 1 article reviews
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human nsclc lines  (ATCC)
96
ATCC human nsclc lines
Human Nsclc Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/10__1158_slash_0008___5472__can___05___1058-34-1-27?v=ATCC
Average 96 stars, based on 1 article reviews
human nsclc lines - by Bioz Stars, 2026-07
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crl 5800 sw1573 atcc  (ATCC)
96
ATCC crl 5800 sw1573 atcc
Crl 5800 Sw1573 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pm36709426-225-28-30?v=ATCC
Average 96 stars, based on 1 article reviews
crl 5800 sw1573 atcc - by Bioz Stars, 2026-07
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human luad cell lines  (ATCC)
94
ATCC human luad cell lines
Human Luad Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pmc11004716-54-0-24?v=ATCC
Average 94 stars, based on 1 article reviews
human luad cell lines - by Bioz Stars, 2026-07
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lung adenocarcinoma  (ATCC)
99
ATCC lung adenocarcinoma
A: Determination of maximum effect (Amax) in 34 cancer cell lines representing multiple cancer lineages including melanoma, pancreatic <t>adenocarcinoma,</t> mesothelioma, non-small cell carcinoma, cholangiocarcinoma, and glioblastoma following 7-days TNG908 treatment. Cell lines are colored by MTAP status. B: Exemplar SDMA immunoblot following a 3-day treatment with TNG908 at cell line-specific EC 20 and EC 50 concentrations from . C: PRMT5 and MTAP levels of cell lines from . D: Correlation of normalized PRMT5 (left) or a single SDMA-modified substrate (right) immunoblot levels at the cell line-specific EC 50 . E: PRMT5 and SDMA immunoblots following treatment a 3-day treatment of TNG908 at 0.02, 0.08, 0.31, 1.25, 5µM in MTAP -deleted LN18 cells.
Lung Adenocarcinoma, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/bio_rxiv__64898__2026__03__26__714409-47-35-61?v=ATCC
Average 99 stars, based on 1 article reviews
lung adenocarcinoma - by Bioz Stars, 2026-07
99/100 stars
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lung cancer  (ATCC)
96
ATCC lung cancer
A: Determination of maximum effect (Amax) in 34 cancer cell lines representing multiple cancer lineages including melanoma, pancreatic <t>adenocarcinoma,</t> mesothelioma, non-small cell carcinoma, cholangiocarcinoma, and glioblastoma following 7-days TNG908 treatment. Cell lines are colored by MTAP status. B: Exemplar SDMA immunoblot following a 3-day treatment with TNG908 at cell line-specific EC 20 and EC 50 concentrations from . C: PRMT5 and MTAP levels of cell lines from . D: Correlation of normalized PRMT5 (left) or a single SDMA-modified substrate (right) immunoblot levels at the cell line-specific EC 50 . E: PRMT5 and SDMA immunoblots following treatment a 3-day treatment of TNG908 at 0.02, 0.08, 0.31, 1.25, 5µM in MTAP -deleted LN18 cells.
Lung Cancer, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pmc05129976-99-10-32?v=ATCC
Average 96 stars, based on 1 article reviews
lung cancer - by Bioz Stars, 2026-07
96/100 stars
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human lung cancer cell lines  (ATCC)
99
ATCC human lung cancer cell lines
A: Determination of maximum effect (Amax) in 34 cancer cell lines representing multiple cancer lineages including melanoma, pancreatic <t>adenocarcinoma,</t> mesothelioma, non-small cell carcinoma, cholangiocarcinoma, and glioblastoma following 7-days TNG908 treatment. Cell lines are colored by MTAP status. B: Exemplar SDMA immunoblot following a 3-day treatment with TNG908 at cell line-specific EC 20 and EC 50 concentrations from . C: PRMT5 and MTAP levels of cell lines from . D: Correlation of normalized PRMT5 (left) or a single SDMA-modified substrate (right) immunoblot levels at the cell line-specific EC 50 . E: PRMT5 and SDMA immunoblots following treatment a 3-day treatment of TNG908 at 0.02, 0.08, 0.31, 1.25, 5µM in MTAP -deleted LN18 cells.
Human Lung Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pmc05015087-158-0-27?v=ATCC
Average 99 stars, based on 1 article reviews
human lung cancer cell lines - by Bioz Stars, 2026-07
99/100 stars
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sw1573 cells  (ATCC)
99
ATCC sw1573 cells
A) Western blot of indicated signaling proteins in <t>SW1573</t> (KRAS G12C /LKB1 WT ) and H1299 (NRAS Q61K /LKB1 WT ) lung cancer cells expressing either scrambled control, HD-PTPshRNA or HD-PTP siRNA. B) Brightfield images of 2D invasion assays of SW1573 and H1299 cells expressing scrambled control and shRNA or siRNA to HD-PTP. C) Growth of cell populations expressing either shRNA or siRNA to HD-PTP. D) Quantitation of invading cells from 2D invasion assays in (B). 2D invasion assays were performed using three biological replicates. Error bars represent the Std. Deviation of Mean. Statistical significance was analyzed using the student’s t-test. * = <.05; ** = <.01. E) Representative images of SW1573 3D spheroids expressing either scrambled control or HD-PTP shRNA and embedded in invasion matrix for the indicated time. Quantification of invasive area in the SW1573 assay in (E). G) Representative images of H1299 spheroids expressing scrambled control or siRNA to HD-PTP and embedded in invasion matrix for the indicated time. H) Quantification of invasive area in the H1299 invasion assay in (G). Quantification of invasive area in H1299 (I) and SW1573 (J) cells expressing scrambled control or shRNA to HD-PTP and treated with vehicle control (DMSO) or the FAK inhibitor defactinib. Quantitative data were analyzed using 4-5 biological replicates. Error bars represent Std. Deviation of Mean and statistical significance was tested using one-way ANOVA, multiple comparisons. * = <.05; ** = <.01; **** = <.0001.
Sw1573 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/bio_rxiv__2023__01__26__525772-140-0-2?v=ATCC
Average 99 stars, based on 1 article reviews
sw1573 cells - by Bioz Stars, 2026-07
99/100 stars
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human lung cancer cell lines ncih460  (JCRB Cell Bank)
90
JCRB Cell Bank human lung cancer cell lines ncih460
A) Western blot of indicated signaling proteins in <t>SW1573</t> (KRAS G12C /LKB1 WT ) and H1299 (NRAS Q61K /LKB1 WT ) lung cancer cells expressing either scrambled control, HD-PTPshRNA or HD-PTP siRNA. B) Brightfield images of 2D invasion assays of SW1573 and H1299 cells expressing scrambled control and shRNA or siRNA to HD-PTP. C) Growth of cell populations expressing either shRNA or siRNA to HD-PTP. D) Quantitation of invading cells from 2D invasion assays in (B). 2D invasion assays were performed using three biological replicates. Error bars represent the Std. Deviation of Mean. Statistical significance was analyzed using the student’s t-test. * = <.05; ** = <.01. E) Representative images of SW1573 3D spheroids expressing either scrambled control or HD-PTP shRNA and embedded in invasion matrix for the indicated time. Quantification of invasive area in the SW1573 assay in (E). G) Representative images of H1299 spheroids expressing scrambled control or siRNA to HD-PTP and embedded in invasion matrix for the indicated time. H) Quantification of invasive area in the H1299 invasion assay in (G). Quantification of invasive area in H1299 (I) and SW1573 (J) cells expressing scrambled control or shRNA to HD-PTP and treated with vehicle control (DMSO) or the FAK inhibitor defactinib. Quantitative data were analyzed using 4-5 biological replicates. Error bars represent Std. Deviation of Mean and statistical significance was tested using one-way ANOVA, multiple comparisons. * = <.05; ** = <.01; **** = <.0001.
Human Lung Cancer Cell Lines Ncih460, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pm27604143-242-0-28?v=JCRB+Cell+Bank
Average 90 stars, based on 1 article reviews
human lung cancer cell lines ncih460 - by Bioz Stars, 2026-07
90/100 stars
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human lung cancer cell lines  (DSMZ)
96
DSMZ human lung cancer cell lines
A) Western blot of indicated signaling proteins in <t>SW1573</t> (KRAS G12C /LKB1 WT ) and H1299 (NRAS Q61K /LKB1 WT ) lung cancer cells expressing either scrambled control, HD-PTPshRNA or HD-PTP siRNA. B) Brightfield images of 2D invasion assays of SW1573 and H1299 cells expressing scrambled control and shRNA or siRNA to HD-PTP. C) Growth of cell populations expressing either shRNA or siRNA to HD-PTP. D) Quantitation of invading cells from 2D invasion assays in (B). 2D invasion assays were performed using three biological replicates. Error bars represent the Std. Deviation of Mean. Statistical significance was analyzed using the student’s t-test. * = <.05; ** = <.01. E) Representative images of SW1573 3D spheroids expressing either scrambled control or HD-PTP shRNA and embedded in invasion matrix for the indicated time. Quantification of invasive area in the SW1573 assay in (E). G) Representative images of H1299 spheroids expressing scrambled control or siRNA to HD-PTP and embedded in invasion matrix for the indicated time. H) Quantification of invasive area in the H1299 invasion assay in (G). Quantification of invasive area in H1299 (I) and SW1573 (J) cells expressing scrambled control or shRNA to HD-PTP and treated with vehicle control (DMSO) or the FAK inhibitor defactinib. Quantitative data were analyzed using 4-5 biological replicates. Error bars represent Std. Deviation of Mean and statistical significance was tested using one-way ANOVA, multiple comparisons. * = <.05; ** = <.01; **** = <.0001.
Human Lung Cancer Cell Lines, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pm27604143-242-0-30?v=DSMZ
Average 96 stars, based on 1 article reviews
human lung cancer cell lines - by Bioz Stars, 2026-07
96/100 stars
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crl 5807  (ATCC)
97
ATCC crl 5807
A) Western blot of indicated signaling proteins in <t>SW1573</t> (KRAS G12C /LKB1 WT ) and H1299 (NRAS Q61K /LKB1 WT ) lung cancer cells expressing either scrambled control, HD-PTPshRNA or HD-PTP siRNA. B) Brightfield images of 2D invasion assays of SW1573 and H1299 cells expressing scrambled control and shRNA or siRNA to HD-PTP. C) Growth of cell populations expressing either shRNA or siRNA to HD-PTP. D) Quantitation of invading cells from 2D invasion assays in (B). 2D invasion assays were performed using three biological replicates. Error bars represent the Std. Deviation of Mean. Statistical significance was analyzed using the student’s t-test. * = <.05; ** = <.01. E) Representative images of SW1573 3D spheroids expressing either scrambled control or HD-PTP shRNA and embedded in invasion matrix for the indicated time. Quantification of invasive area in the SW1573 assay in (E). G) Representative images of H1299 spheroids expressing scrambled control or siRNA to HD-PTP and embedded in invasion matrix for the indicated time. H) Quantification of invasive area in the H1299 invasion assay in (G). Quantification of invasive area in H1299 (I) and SW1573 (J) cells expressing scrambled control or shRNA to HD-PTP and treated with vehicle control (DMSO) or the FAK inhibitor defactinib. Quantitative data were analyzed using 4-5 biological replicates. Error bars represent Std. Deviation of Mean and statistical significance was tested using one-way ANOVA, multiple comparisons. * = <.05; ** = <.01; **** = <.0001.
Crl 5807, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pmc07308074-147-7-5?v=ATCC
Average 97 stars, based on 1 article reviews
crl 5807 - by Bioz Stars, 2026-07
97/100 stars
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lines  (ATCC)
95
ATCC lines
A) Western blot of indicated signaling proteins in <t>SW1573</t> (KRAS G12C /LKB1 WT ) and H1299 (NRAS Q61K /LKB1 WT ) lung cancer cells expressing either scrambled control, HD-PTPshRNA or HD-PTP siRNA. B) Brightfield images of 2D invasion assays of SW1573 and H1299 cells expressing scrambled control and shRNA or siRNA to HD-PTP. C) Growth of cell populations expressing either shRNA or siRNA to HD-PTP. D) Quantitation of invading cells from 2D invasion assays in (B). 2D invasion assays were performed using three biological replicates. Error bars represent the Std. Deviation of Mean. Statistical significance was analyzed using the student’s t-test. * = <.05; ** = <.01. E) Representative images of SW1573 3D spheroids expressing either scrambled control or HD-PTP shRNA and embedded in invasion matrix for the indicated time. Quantification of invasive area in the SW1573 assay in (E). G) Representative images of H1299 spheroids expressing scrambled control or siRNA to HD-PTP and embedded in invasion matrix for the indicated time. H) Quantification of invasive area in the H1299 invasion assay in (G). Quantification of invasive area in H1299 (I) and SW1573 (J) cells expressing scrambled control or shRNA to HD-PTP and treated with vehicle control (DMSO) or the FAK inhibitor defactinib. Quantitative data were analyzed using 4-5 biological replicates. Error bars represent Std. Deviation of Mean and statistical significance was tested using one-way ANOVA, multiple comparisons. * = <.05; ** = <.01; **** = <.0001.
Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsclc+cell+lines+sw1573/pm30355485-239-43-53?v=ATCC
Average 95 stars, based on 1 article reviews
lines - by Bioz Stars, 2026-07
95/100 stars
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Image Search Results


A: Determination of maximum effect (Amax) in 34 cancer cell lines representing multiple cancer lineages including melanoma, pancreatic adenocarcinoma, mesothelioma, non-small cell carcinoma, cholangiocarcinoma, and glioblastoma following 7-days TNG908 treatment. Cell lines are colored by MTAP status. B: Exemplar SDMA immunoblot following a 3-day treatment with TNG908 at cell line-specific EC 20 and EC 50 concentrations from . C: PRMT5 and MTAP levels of cell lines from . D: Correlation of normalized PRMT5 (left) or a single SDMA-modified substrate (right) immunoblot levels at the cell line-specific EC 50 . E: PRMT5 and SDMA immunoblots following treatment a 3-day treatment of TNG908 at 0.02, 0.08, 0.31, 1.25, 5µM in MTAP -deleted LN18 cells.

Journal: bioRxiv

Article Title: Identification of a lineage-agnostic splicing signature caused by PRMT5 inhibition

doi: 10.64898/2026.03.26.714409

Figure Lengend Snippet: A: Determination of maximum effect (Amax) in 34 cancer cell lines representing multiple cancer lineages including melanoma, pancreatic adenocarcinoma, mesothelioma, non-small cell carcinoma, cholangiocarcinoma, and glioblastoma following 7-days TNG908 treatment. Cell lines are colored by MTAP status. B: Exemplar SDMA immunoblot following a 3-day treatment with TNG908 at cell line-specific EC 20 and EC 50 concentrations from . C: PRMT5 and MTAP levels of cell lines from . D: Correlation of normalized PRMT5 (left) or a single SDMA-modified substrate (right) immunoblot levels at the cell line-specific EC 50 . E: PRMT5 and SDMA immunoblots following treatment a 3-day treatment of TNG908 at 0.02, 0.08, 0.31, 1.25, 5µM in MTAP -deleted LN18 cells.

Article Snippet: Human cancer cell lines: skin cutaneous melanoma (SK-MEL-5, A101D, Hs294T, IGR-1, SH-4), glioblastoma (LN18, GB-1, U-87 MG, AM-38, KS-1, A172), pancreatic adenocarcinoma (Panc03.27, Panc10.05, SU.86.86, BxPC-3, Miapaca2), mesothelioma (ONE58, SDM103T2, NCI-H2052, ZL5, ACC-MESO-4), cholangiocarcinoma (KKU-100), lung adenocarcinoma (A549, NCI-H1373, NCI-H520, SK-LU-1, HARA, NCI-H322, HCC-1588, SW-900, HCC4006, NCI-H1650, LU99, SW1573), colorectal adenocarcinoma (HCT116) and chronic myeloid leukemia (HAP-1) cells were obtained from ATCC (Manassas, VA) and cultured in cell dependent media (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT).

Techniques: Western Blot, Modification

A: Heatmap of 22 distinct alternative splicing events (ASE) following 3-days TNG908 treatment in the MTAP -deleted GBM LN-18 cell line. Data reported as percent spliced in (PSI) of the alternative exons (% inclusion = inclusion/sum of inclusion + exclusion). Table of PSI values transformed, plotted, and expressed as LogIC 50 values. B: Heatmap of 22 ASEs expressed as PSI following 3-day treatment of 1µM TNG908 in LN-18 MTAP -WT or LN-18 MTAP -deleted cells. C: Heatmap of 22 distinct ASEs from MTAP -deleted cells treated for 3-days with treated with 1µM TNG908, the maximally efficacious dose identified from Figure 2.1. Data reported as ΔPSI of the alternative exons (ΔPSI = PSI DMSO – PSI TNG908). Cell lines represent glioblastoma (GBM), colorectal cancer (CRC), non-small cell lung cancer (NSCLC), and pancreatic adenocarcinoma (PDAC). D: ΔPSI of RPAIN ASE for treated cell lines.

Journal: bioRxiv

Article Title: Identification of a lineage-agnostic splicing signature caused by PRMT5 inhibition

doi: 10.64898/2026.03.26.714409

Figure Lengend Snippet: A: Heatmap of 22 distinct alternative splicing events (ASE) following 3-days TNG908 treatment in the MTAP -deleted GBM LN-18 cell line. Data reported as percent spliced in (PSI) of the alternative exons (% inclusion = inclusion/sum of inclusion + exclusion). Table of PSI values transformed, plotted, and expressed as LogIC 50 values. B: Heatmap of 22 ASEs expressed as PSI following 3-day treatment of 1µM TNG908 in LN-18 MTAP -WT or LN-18 MTAP -deleted cells. C: Heatmap of 22 distinct ASEs from MTAP -deleted cells treated for 3-days with treated with 1µM TNG908, the maximally efficacious dose identified from Figure 2.1. Data reported as ΔPSI of the alternative exons (ΔPSI = PSI DMSO – PSI TNG908). Cell lines represent glioblastoma (GBM), colorectal cancer (CRC), non-small cell lung cancer (NSCLC), and pancreatic adenocarcinoma (PDAC). D: ΔPSI of RPAIN ASE for treated cell lines.

Article Snippet: Human cancer cell lines: skin cutaneous melanoma (SK-MEL-5, A101D, Hs294T, IGR-1, SH-4), glioblastoma (LN18, GB-1, U-87 MG, AM-38, KS-1, A172), pancreatic adenocarcinoma (Panc03.27, Panc10.05, SU.86.86, BxPC-3, Miapaca2), mesothelioma (ONE58, SDM103T2, NCI-H2052, ZL5, ACC-MESO-4), cholangiocarcinoma (KKU-100), lung adenocarcinoma (A549, NCI-H1373, NCI-H520, SK-LU-1, HARA, NCI-H322, HCC-1588, SW-900, HCC4006, NCI-H1650, LU99, SW1573), colorectal adenocarcinoma (HCT116) and chronic myeloid leukemia (HAP-1) cells were obtained from ATCC (Manassas, VA) and cultured in cell dependent media (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT).

Techniques: Alternative Splicing, Transformation Assay

A: Determination of maximum effect (Amax) in 34 cancer cell lines representing multiple cancer lineages including melanoma, pancreatic adenocarcinoma, mesothelioma, NSCLC, cholangiocarcinoma, and glioblastoma following 7-days TNG908 treatment. Cell lines are colored by MTAP status. B: Normalized SDMA levels following a 1-day exogenous administration of MTA in HAP1 MTAP -WT cells.

Journal: bioRxiv

Article Title: Identification of a lineage-agnostic splicing signature caused by PRMT5 inhibition

doi: 10.64898/2026.03.26.714409

Figure Lengend Snippet: A: Determination of maximum effect (Amax) in 34 cancer cell lines representing multiple cancer lineages including melanoma, pancreatic adenocarcinoma, mesothelioma, NSCLC, cholangiocarcinoma, and glioblastoma following 7-days TNG908 treatment. Cell lines are colored by MTAP status. B: Normalized SDMA levels following a 1-day exogenous administration of MTA in HAP1 MTAP -WT cells.

Article Snippet: Human cancer cell lines: skin cutaneous melanoma (SK-MEL-5, A101D, Hs294T, IGR-1, SH-4), glioblastoma (LN18, GB-1, U-87 MG, AM-38, KS-1, A172), pancreatic adenocarcinoma (Panc03.27, Panc10.05, SU.86.86, BxPC-3, Miapaca2), mesothelioma (ONE58, SDM103T2, NCI-H2052, ZL5, ACC-MESO-4), cholangiocarcinoma (KKU-100), lung adenocarcinoma (A549, NCI-H1373, NCI-H520, SK-LU-1, HARA, NCI-H322, HCC-1588, SW-900, HCC4006, NCI-H1650, LU99, SW1573), colorectal adenocarcinoma (HCT116) and chronic myeloid leukemia (HAP-1) cells were obtained from ATCC (Manassas, VA) and cultured in cell dependent media (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT).

Techniques:

A) Western blot of indicated signaling proteins in SW1573 (KRAS G12C /LKB1 WT ) and H1299 (NRAS Q61K /LKB1 WT ) lung cancer cells expressing either scrambled control, HD-PTPshRNA or HD-PTP siRNA. B) Brightfield images of 2D invasion assays of SW1573 and H1299 cells expressing scrambled control and shRNA or siRNA to HD-PTP. C) Growth of cell populations expressing either shRNA or siRNA to HD-PTP. D) Quantitation of invading cells from 2D invasion assays in (B). 2D invasion assays were performed using three biological replicates. Error bars represent the Std. Deviation of Mean. Statistical significance was analyzed using the student’s t-test. * = <.05; ** = <.01. E) Representative images of SW1573 3D spheroids expressing either scrambled control or HD-PTP shRNA and embedded in invasion matrix for the indicated time. Quantification of invasive area in the SW1573 assay in (E). G) Representative images of H1299 spheroids expressing scrambled control or siRNA to HD-PTP and embedded in invasion matrix for the indicated time. H) Quantification of invasive area in the H1299 invasion assay in (G). Quantification of invasive area in H1299 (I) and SW1573 (J) cells expressing scrambled control or shRNA to HD-PTP and treated with vehicle control (DMSO) or the FAK inhibitor defactinib. Quantitative data were analyzed using 4-5 biological replicates. Error bars represent Std. Deviation of Mean and statistical significance was tested using one-way ANOVA, multiple comparisons. * = <.05; ** = <.01; **** = <.0001.

Journal: bioRxiv

Article Title: Loss of the endocytic tumor suppressor HD-PTP phenocopies LKB1 and promotes RAS-driven oncogenesis

doi: 10.1101/2023.01.26.525772

Figure Lengend Snippet: A) Western blot of indicated signaling proteins in SW1573 (KRAS G12C /LKB1 WT ) and H1299 (NRAS Q61K /LKB1 WT ) lung cancer cells expressing either scrambled control, HD-PTPshRNA or HD-PTP siRNA. B) Brightfield images of 2D invasion assays of SW1573 and H1299 cells expressing scrambled control and shRNA or siRNA to HD-PTP. C) Growth of cell populations expressing either shRNA or siRNA to HD-PTP. D) Quantitation of invading cells from 2D invasion assays in (B). 2D invasion assays were performed using three biological replicates. Error bars represent the Std. Deviation of Mean. Statistical significance was analyzed using the student’s t-test. * = <.05; ** = <.01. E) Representative images of SW1573 3D spheroids expressing either scrambled control or HD-PTP shRNA and embedded in invasion matrix for the indicated time. Quantification of invasive area in the SW1573 assay in (E). G) Representative images of H1299 spheroids expressing scrambled control or siRNA to HD-PTP and embedded in invasion matrix for the indicated time. H) Quantification of invasive area in the H1299 invasion assay in (G). Quantification of invasive area in H1299 (I) and SW1573 (J) cells expressing scrambled control or shRNA to HD-PTP and treated with vehicle control (DMSO) or the FAK inhibitor defactinib. Quantitative data were analyzed using 4-5 biological replicates. Error bars represent Std. Deviation of Mean and statistical significance was tested using one-way ANOVA, multiple comparisons. * = <.05; ** = <.01; **** = <.0001.

Article Snippet: SW1573 cells (ATCC) were grown in L-15(ATCC 30-2008) medium with 10% FBS, 2mM l-glutamine, and 1% penicillin-streptomycin.

Techniques: Western Blot, Expressing, Control, shRNA, Quantitation Assay, Invasion Assay